A clinical trial was conducted on a collagen peptide that contains a high amount of active ingredients and can be transferred into the blood more.

DI-PEPTIDE, which contains high levels of active ingredients Pro-Hyp (PO) and Hyp-Gly (OG), improved skin moisture, smoothness and clarity compared to standard type product. Furthermore, only in DI-PEPTIDE, the elasticity significantly increased and number of wrinkles significantly decreased. This is probably because the active ingredients PO and OG acted on skin moisturizing factors, elasticity factors and metabolic factors.

Reference：Inoue N, Sugihara F, Wang X: Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomized double-blind placebo-controlled clinical study. J Sci Food Agric. 96(12), 4077-81, 2016

UV spots will eventually come to the surface and become new blemish.

Reference：Sugihara F, Inoue N: Clinical effects of collagen hydrolysates ingestion on UV-induced pigmented spots of human skin: A preliminary study. Health Sciences; 28:153-156, 2012

Change = Week 6 - Baseline or Week 12 – Baseline, Unit：Arbitrary Unit、Mean ± SD

As a post-hoc test for repeated measures two-dimensional array analysis, Tukey test was performed to compare with Placebo group. ITT analysis.

Moisture：Corneometer CM825、Elasticity：Cutometer MPA580

Reference：Koizumi S, Inoue N, Shimizu M, Chang-ju Kwon, Hwa-young Kim, Kyoung Sik Park; Effects of Dietary Supplementation with Fish Scales-Derived Collagen Peptides on Skin Parameters and Condition: A Randomized, Placebo-Controlled, Double-Blind Study; International Journal of Peptide Research and Therapeutics, 1-6, 2017

Ingestion of 3 g / day of Standard type collagen peptide significantly improved skin moisture content and elasticity. This is probably because the active ingredients Pro-Hyp (PO) and Hyp-Gly (OG) contained in this collagen peptide acted on skin moisturizing factors, elasticity factors and metabolic factors.

Mean ± SE, AU：Arbitary units

Those results were performed post-hoc testing using Tukey test for Two-way ANOVA, #p<0.05, ##p<0.01 VS Placebo,

Paired, t-test,  *p<0.05, **p<0.01 VS Baseline

Reference : Sugihara F, Inoue N, Wang X: Clinical effects of ingesting collagen hydrolysate on facial skin properties. Japanese pharmacology and Therapeutics, 43:67-70, 2015

DI-PEPTIDE group showed significantly higher moisture content and elasticity than the placebo group from week 4, and skin roughness significantly decreased from week 4. This is probably because the active ingredients PO and OG acted on the skin's moisturizing, and epidermal turnover factors. 

Mean ± SE,  *p<0.05 VS Baseline

Two-sided test, and the significance level was set to 5%, with a confidence interval of 95%. Wilcoxon signed-rank test was used for intragroup comparisons of baseline and after 3 months of intake.

Reference : Himeno, A., Tsujikami, M., Koizumi, S., Watanabe, T., & Igase, M., Effect of Reducing Pigmentation by Collagen Peptide Intake: A Randomized, Double-Blind, Placebo-Controlled Study. Dermatology and therapy, 2022, 10/1007/s13555-022-00748-4.

https://doi.org/10.1007/s13555-022-00748-4

After taking 5g/day of DI-PEPTIDE or Placebo for three months, pigmented patches（Level 3: Large, dark spots） in the DI-PEPTIDE group was significantly reduced, whereas those in the placebo group was not significantly reduced compared with their baseline.