8-1.  Clinical studies

[ Clinical Trial Conditions ]

 ● Open trial (before and after comparison)
 ● Age 24 - 61 (40s - 50s 77%)
 ● 26 subjects
 ● Collagen peptide 5 g/day, 10 weeks (70 days)
 ● Evaluation : Body composition analysis, 2-step test* (locomotive performance), Interview

*2-step test：

An easy way to examine Locomotive syndrome, made by the Japanese Orthopaedic Association. By examining the step size and hight, evaluating the muscle strength, balance ability, and flexibility of the lower limbs.

Mean±SE, ~{#} p<0.05 (vs 0week)

Muscle rate

Increased 0.5%

2-step test

Decreased pre-locomotive syndrome people (35 to 23%)

Interview

Going up and down the stairs got easier. Increased flexibility of the body. etc.

Reference：7th Conference of Japanese Society for Amino Acids Sciences, poster presentation

8-2.  Mice research data

Active ingredients reaching muscles

It was confirmed that Pro-Hyp (PO) and Hyp-Gly (OG) reached blood and muscle tissues in about 0.5 to 3 hours after ingestion of TYPE-M.

Plasma Gastroc
nemius		 Soleus Extensor
digitorum longus
Maximum PO
(time after
administration)		 28 μM
(3h)		 20 μM
(3h)		 40 μM
(3h)		 16 μM
(3h)
Maximum OG
(time after
administration)		 9 μM
(1h)		 5 μM
(3h)		 9 μM
(3h)		 3 μM
(0.5h)

Reference：10th Conference of Japanese Society for Amino Acids Sciences, presentation

8-3.  Effect on muscle differentiation

PO : Pro-Hyp、OG：Hyp-Gly

  1. Active ingredients PO and OG reach muscle tissue
  2. Expression of Myogenin, MyoD, and IGF-1 genes is upregulated via mTOR
  3. The short diameter of myoblasts becomes thicker
     

Reference:  Kitakaze T, Sakamoto T, Kitano T, Inoue N, Sugihara F, Harada N, Yamaji R, The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy, Biochem Biophys Res Commun., 478 (3) :1292-7, 2016

VideoIncreasing Muscle Density