We provide multiple types of collagen products. Please select the optimum product for your experimental use. Typical culture methods using collagen are as follows.
Prepare the following three types of solutions, i.e, A, B, and C.
A: Cellmatrix Type I-A or I-P
B: 10x concentrated Culture Solution *
C: Buffer Solution for Reconstitution
Mix A and B well at a ratio of 8 to 1 while cooling A and B so that the mixture does not foam. Then add C at a ratio of 1 to previous mixture and mix them together. *
* In the case of using culture solution at a concentration of approximately 5x,the following mixture ratio will apply: A:B:C=7:2:1
This is a method for three-dimensional cell culture in a collagen gel. In 3D culture, cells can be cultured in an environment extremely similar to the natural in vivo conditions.
Add the resultant mixture (A+B+C) mentioned above to cell pellet obtained after centrifugation and mix well.
Dispense this collagen mixture solution to a culture dish and leave the solution at 37°C for 30 minutes so that the solution will turn into a gel.
After the gel is formed, overlay with culture solution and perform culture in an ordinary manner.
Cellmatrix Type I-A is a collagen product with an optimized gelation rate and transparency for 3-dimensional culture. It can quickly form a gel before the cells sink to the bottom of the culture dish. In addition, the gel is highly transparent, allowing easy observation of the cells embedded in the collagen gel.
This method involves cell culture on a collagen gel. Culturing cells on a collagen gel mimics an environment similar to the living body.
The above collagen mixture solution will turn into a gel when the solution is dispensed to a culture dish and heated at 37°C for 30 minutes. Keep cold the mixture before incubation or during mixing.
After gelation spread the cell dispersion onto the gel. Once the cells adhere to the gel, it will be possible to culture the cells like normal monolayer culture.
Recommended product: Cellmatrix Type I-A and Type I-P
In this method, cells are cultured on substrates coated with collagen molecules to improve cell adhesion and activation of intracellular signals by contact with collagen molecules.
Recommended product: Cellmatrix Type I-C and Type I-P
Each Cellmatrix product has a different gelling ability and viscosity. For culturing on a collagen gel, please use products with high gelation ability. Products with lower viscosity are suitable for coating culture because they are easier to handle. Please refer to the summarized table below.
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For Research Use Only
The above products are sold for research purpose only. Regardless of purposes, such as production of drugs, product quality control, various diagnostic test, medical treatment, or other researches, do not use products for human body.
