beMatrix gelatin has already been adopted as a raw material for a medical device approved by the FDA. It has also been considered as a raw material for new products from many companies both in Japan and overseas.

The average molecular weight is different. Because LS-H is a high molecular weight product and has high gel strength, it is suitable for preparation of hydrogels for DDS and cell transplantation. However, LS-W is a low molecular weight product with low viscosity, and is suitable for coating the material surface. In addition, by mixing these two products, it is also possible to customize physical properties suitable for each user's application.

We can submit COA, certificate of origin, etc., according to your request. Please contact us for further information.

beMatrix collagen AT was used as the culture material in the 1st clinical study of iPS cell-derived tissue transplantation conducted by Dr. Masayo Takahashi. In addition, some groups are considering its use in clinical research.

The raw material and extraction methods are different. Collagen AT is an acid extracted collagen derived from porcine tendons. However, collagen TE is extracted from porcine skin using enzymatic methods.

Please choose an optimal product suitable for your experiments. The features of each product are summarized below. If you plan to perform collagen gel embedded culture (3D culture), we recommend Cellmatrix Type I-A.

 

Cellmatrix	Gelling ability	Coating
Type Ⅰ-A	Very good	Good
Type Ⅰ-P	Good	Good
Type Ⅰ-C	Not good	Very good
Type Ⅲ	Not good	Very good
Type Ⅳ		Very good

Cellmatrix Type I-A is recommended. It is the most suitable product for collagen gel embedded culture because of its rapid gelation rate and high gel transparency.

Cellmatrix Type I-C is recommended. It is a product with the lowest viscosity among our Type I collagen products and is suitable for collagen coating culture. 

The above two products cannot form a gel by themselves. However, they can be used in collagen gel culture by mixing them with Cellmatrix Type I--A. Stable gels can be formed if the ratio of Cellmatrix Type I-A is 40% or more.

Dilution with dilute hydrochloric acid solution at pH 3.0 is recommended. If foam formation occurs during the dilution process, please leave overnight at 4°C and then remove the foam. In case of time constraints, defoaming can also be done by centrifugation.

Gels can be prepared by placing the gelatin solution in a low temperature environment. However, as it is not crosslinked, the gel promptly liquefies if transferred to a culture environment at 37°C. To perform cell culture on a gelatin gel, crosslinking treatment must be performed beforehand using glutaraldehyde, genipin, or similar reagents.